硝苯吡啶和维拉帕米治疗肝纤维化细胞学研究

A CELLULAR STUDY OF NIFEDIPINE AND VERAPAMIL IN TREATMENT OF HEPATOFIBROSIS

目的研究硝苯吡啶（ｎｉｈｅｄｉｐｉｎｅｃ，Ｎｉｆ）和维拉帕米（ｖｅｒａｐａｍｉｌ，Ｖｅｒ）对人胚肺成纤维细胞（ｈｕｍａｎｌｕｎｇｆｉｂｒｏｂｌａｓｔｓ，ＨＬＦ）的ＤＮＡ和胶原合成的影响，从细胞水平评价钙拮抗剂防治器官纤维化并提供实验依据。方法采用ＭＴＴ比色法、３Ｈ－ＴｄＲ和３Ｈ－脯氨酸掺入法测定细胞增殖、ＤＮＡ合成和胶原合成。结果Ｎｉｆ和Ｖｅｒ在０～５０μｍｏｌ／Ｌ浓度范围内均以浓度依赖方式抑制ＨＬＦ增殖、ＤＮＡ合成及胶原合成（Ｐ＜０．０５～０．０１）。

Objective To investigate the potentiality of using calcium antagonists including nifedipinec(Nif) and verapamil(Ver) on the proliferation, DNA and collagen syntheses of human lung fibroblasts(HLF).[WT5”HZ]Methods The cell proliferation was determined by the method of NTT, measuring the incorporation of 3HTdR and 3HLproline.Results The results showed that both Nif and Ver suppressed HLF proliferation, DNA and collagen syntheses in drug concentrationdependent manner at 10￣40 μmol.L1(P<0.05￣0.01).Conclusion Nif and Ver were hopeful drugs for antifibrosis.