钒酸氧化法测定血清胆红素的实验评价

Experimental evaluation of serum bilirubin determination with vanadic acid oxidation method

目的对钒酸氧化法测定血清胆红素进行方法学评价。方法收集40例高、中、低浓度的血清样本进行钒酸氧化法与重氮法的比对,分析钒酸氧化法的精密度、灵敏度、稳定性并做干扰实验。结果钒酸氧化法测定血清总胆红素(TBil)批内变异系数(CV)为1.58%,批间CV为2.94%;血清直接胆红素(DBil)批内CV为1.23%,批间CV为2.7%;平均回收率TBil为101.1%,DBil为100.1%。维生素C(Vit C)≤0.5 g/L时对钒酸氧化法和重氮法均无干扰,三酰甘油≤6 mmol/L时对钒酸氧化法无干扰,对重氮法有明显干扰。血红蛋白浓度≤8 g/L时对钒酸氧化法无干扰,对重氮法有较大干扰。结论钒酸氧化法测定TBil和DBil是较理想的方法。

Objective To evaluate the method of serum bilirubin determination with vanadic acid oxidation assay. Methods 40 serum samples of high, middle and low bilirubin concentration were determined by vanadic acid oxidation assay and compaired with diazo method. The precision, sensitivity, stability and interference of vanadic acid oxidation as-say were analysed. Results The within-run and between-run coefficient of variation （CV） of total bilirubin （TBil） by vanadic acid oxidation assay were 1.58% and 2.94%, The within-run and between-run CV of direct bilirubin（DBil） were 1.23% and 2.7%. The average rate of recovery of TBil and DBil were 101, 1% and 100. 1%. When vitamin C concentration was≤0.5 g/L ,both vanadic acid oxidation assay and diazo method had no interference. When triglyceride was≤6 mmol/L,the vanadic acid oxidation assay had no interference and the diazo method had significant interference. When hemoglobin was≤8 g/L, the vanadie acid oxidation assay had no interference and the diazo method had significant interference. Conclusions Vanadic acid oxidation assay is an ideal method for TBil and DBil determination.