依达拉奉对脑缺血再灌注大鼠海马一氧化氮的影响

Effects of Edaravone on NO concentration in rat hippocampus with transient cerebral ischemia and reperfusion

目的研究依达拉奉对脑缺血再灌注大鼠海马一氧化氮(NO)产生的影响。方法大鼠脑缺血采用四血管阻断法,选择性测定电极检测的浓度。实验分为生理盐水组、依达拉奉组(Edaravone)和7-Nitroindazole(7-NI)组。结果依达拉奉和7-NI皆未影响大鼠的血压和海马血流量,均显著减少了缺血再灌注时海马内NO的产生(均P<0.001)。结论依达拉奉可能通过抑制神经型一氧化氮合酶(nNOS)或减少NO而起到神经保护作用。

Objective To investigate the effects of Edaravone on nitric oxide（NO）concentration in rat hippoeampus after transient cerebral isehemia. Methods The model of cerebral isehemia was made by 4-vessel occlusion for 10min. NO concentration was measured directly by an NO-selective electrode method. Saline,Edaravone or 7-Nitroindazole（7-NI） were administered intraperitoneally 20 min before the onset of isehemia. Results Both Edaravone and 7-NI did not affect the hippoeampal blood flow and mean arterial blood pressure （MABP）, compared with saline group. Edaravone and 7-NI significantly reduced the NO production during postisehemie early reperfusion （P〈0. 01）. Conclusion Edaravone has protective effect on hippoeampal neurons against cerebral isehemia-reperfusion injury by reducing the activity of neuronal NO synthase （nNOS） or reducing the content of NO in hippoeampus of rat.