促红细胞生成素对急性脑损伤的保护作用

Protection of erythropoietin for acute brain injury

目的观察重组人促红细胞生成素(r-Epo)对大鼠急性脑损伤后脑细胞凋亡及脑水肿的影响。方法30只大鼠分为A、B、C 3组,B、C 2组用自由落体打击器制作脑外伤模型,C组r-Epo预处理,48 h后断头取脑。制作石蜡切片,TUNEL法检测细胞凋亡,同时用干湿重法测定各组大鼠脑组织含水量。结果与B组比较,C组外伤侧脑组织含水量明显降低(P<0.01),细胞凋亡数明显减少(P<0.01)。结论r-Epo能显著降低急性脑损伤后脑水肿,减少水肿区神经细胞凋亡,对急性损伤后脑组织有良好的保护作用。

Objective To investigate the effects of erythropoietin on neural cells and brain edema in rats after acute injury. Methods 30 rats were divided into three groups, group A, B, and C. The models of acute brain injury of group B and C rats were established with a free-falling device and 10 rats of group B were injected into erythropoietin in advance. All their brains were removed 48 hours later. Detected neuron apoptosis condition with TUNEL method, the percentage of water in the brain tissue was measured with dry-wet method. Results Contrasted the percentage of water in the brain tissue of injure side and the number of neuron apoptosis, group C was notable lower than group B. Conclusion Erythropoietin can lighten brain edema, resist neuron apoptosis, and has positive effect on injure brain tissue.