已烯雌酚对蝌蚪抗敌敌畏诱导氧化损伤的作用研究

The Effect of Diethylstilbestrol on Antioxidation of Tadpoles Exposed to Dichlorovos

研究已烯雌酚（DES）对中华大蟾蜍（Bufobufo gargarizans）蝌蚪抗敌敌畏（0，0-二甲基-0-（2，2-二氯乙烯基）磷酸酯，DDVP）诱导的氧化损伤的作用．方法：蝌蚪（30～35期）随机分为5组：I（0mg／LDDVP＋0mg／LDES）为空白组；Ⅱ（12．9mg／LDDVP＋0mg／LDES）为单独DDVP处理组；Ⅲ（12．9mg／LDDVP＋1．0mg／LDES）；1V（12．9mg／LDDVP＋2．0mg／LDES）；V（12．9mg／LDDVP＋3．0mg／LDES）为DDVP和DES联合处理组．在染毒24h和72h时测定蝌蚪肝组织中超氧化物歧化酶（SoD）和谷胱甘肽过氧化物酶（GSH-Px）的活性以及丙二醛（MDA）的含量，计算抗氧化指数．其结果在DDVP（12．9mg／L）存在的条件下，随着DES（1．0mg／L）的加入和浓度的依次加大（2．0mg／L，3．0mg／L），SOD活性呈下降趋势，72h时具显著性（P〈0．05）；GSH—Px活性呈先升高后降低的变化趋势，在24h和72h均具极显著性（P〉0．01）；MDA含量呈先降低后升高的变化趋势，72h时具显著性（P〈0．05）。其中联合处理组Ⅲ的抗氧化能力最强．结论：DES对DDVP诱导的氧化损伤作用具有拮抗效应，能有效地增强中华大蟾蜍蝌蚪的抗氧化能力．

The effect of diethylstilbestrol （DES） on antioxidation in tadpoles （Bufo bufo gargarizans） exposed to dichlorovos （0,0 Dimethyl-0-0 （2,2-Dichloro ethylene） Phosphate,DDVP） was studied. Methods：Tadpoles （30-35 tadpole stage） were randomly divided into 5 groups, as control group I （0 mg/L DDVP＋0 mg/L DES）, single DDVP group Ⅱ （12.9 mg/L DDVP＋0 mg/L DES）, experimental groups Ⅲ （12. 9 mg/L DDVP＋I. 0 mg/L DES ）, Ⅳ （12.9 mg/L DDVP＋2.0 mg/L DES）and V（12.9 mg/L DDVP＋3.0 mg/L DES）. The activities of superoxide dismutase and glutathione peroxidase （GSH-Px） and the content of malondialdehyde （MDA） in liver of tadpoles were measured after 24 h and 72 h exposure, and the antioxidant index was computed. Results. With DDVP being 12.9 mg/L, the activity of SOD de- creased as DES was added and its concentration increased. After 72 h exposure, the activities of SOD reached the significant level（P〈0.05）. The activities of GSH-Px increased at the first stage and then decreased, indicating the most significant difference（P〈0.01） at 24 h and 72 h exposure. The MDA accumulation which showed the adverse tendency as GSH-Px did, indicated the significant difference（P〈0.05）at 72 h exposure. The experimental group Ⅲ had the strongest antioxidation. Conclusion： DES has the strongest antagonistic effect on oxidative damages induced by DDVP, which can enhance antioxidation in tadpoles of Bu fo bu fo gargarizans effectively.