直肠癌K-ras基因突变和细胞动力学异常

Mutant K-ras and Abnormal Cytokinetics in Rectal Cancer

采用扩增片段限制性长度多态性分析（Amp－RFLP）和流式细胞分析技术（FCM）对直肠癌切除标本、癌旁远端肉眼观察“正常”的肠组织及正常肠粘膜，结合病理资料，比较其细胞DNA倍体性、S期百分比和增殖指数，检测K－ras基因12位密码子点突变，结果表明：直肠癌是进展迅速的恶性肿瘤，细胞增殖活性异常升高；K－ras基因突变是伴随直肠癌的一种基因异常表现，癌旁组织出现基因水平的异常发生在组织形态学和细胞动力学改变之前，是癌变发生的早期行为。

With close relevance to pathological data. 36 resected specimens of human rectal carcinoma, 33 adjacent tissues and 31 normal colon mucosa were studied for cell kinetics and point mutation in codon 12 of K-ras by means of AmpRFLP and flow cytometry. As a result it was indicated that rectal cancer can be categorized as a tumor with rapid progression. Extraordinary activity of cell proliferation and mutation of K-ras gene were common manifestations of rectal cancer.The change of K-ras gene which aften occurred prior to the changes of histomorphology and cell kinetics abnormality was the earliest manifestation in cellular transformation toward malignancy.