顺氨氯铂、阿霉素增强人抗CD_3单克隆抗体活化杀伤细胞的杀瘤效应

Enhancement of CD_3AK Cell-mediated Cytotoxicity by Anticancer Agents CDDP or ADM

应用抗CD3单克隆抗体和基因重组人IL-2共同诱导人外周血单个核细胞，制备抗CD3单克隆抗体活化的杀伤细胞（CD3AK）。利用LDH释放法，观察顺氨氯铂（CDDP）和阿霉素（ADM）预处理人肝癌细胞系H－7402，对CD3AK杀伤该靶细胞的调节作用。ABC－ELISA法检测CDDP和ADM对H-7402细胞表达细胞间粘附分子-1（ICAM-1）、HLA－ABC、HLA－DR抗原的影响。结果表明：经CDDP（2．0μg／ml）、ADM（O．1μg／ml）预处理12h的H－7402细胞对CD3AK的杀伤敏感性显著提高（P＜0.05）；CDDP能够明显促进H－7402细胞表达ICAM-1、HLA－ABC分子（P＜0.05）；ADM预处理的肿瘤细胞表面三种分子的表达均未发生明显的变化。CDDP促进人肝癌细胞对CD3AK的杀伤敏感性可能与其上调了肿瘤细胞表面的ICAM-1分子有关。

Human CD3AK cells were prepared from peripheral blood mononuclear cells by culturing them in recombinant IL-2 and anti CD3McAb. The vulnerabi lity H-7402 pretreated with CDDP or ADM to lysis by CD3AK, and the expression of ICAM-1, HLA-ABC, HLA-DR antigen induced by CDDP or ADM were examined. The result showed that pretreatment of H-7402 cells with CDDP (2ug/ml) or ADM (0. 1ug/ml) for 12 hrs resulted in increased vulnerability of these cells to CD,AK-mediated killing (P<0. 05). The expression of ICAM-1, HLA-ABC but not HLA-DR on H-7402 cells were increased after treatment of the tumor cells with CDDP (P<0. 05). However, ADM showed no regulation effect on the expression of the surface antigens on H-7402 cells. The results suggest that enhancement of CD3AK-mediated cytotoxicity by CDDP may probably be correlated with the modulation of ICAM-1 molecule on H-7402 cells.