对体外培养鸡听壶支持细胞增殖的观察

Supporting cells proliferation of the chick lagena epithelium in vitro culture

选用出生后７～１４ｄ罗曼鸡１５只，解剖出完整耳蜗进行体外培养，３ｄ后在培养液中加入氚标胸腺嘧啶核苷（３ＨＴｄＲ），分别在培养第６ｄ、第９ｄ终止培养，切片曝光观察。结果显示：加３ＨＴｄＲ后培养３ｄ、６ｄ的听壶上皮均有少量支持细胞增殖，被标记的支持细胞多位于基底层及中层，少数移至表面。提示：启动或刺激支持细胞增殖的因素可能在分离出来的耳蜗上皮中。

?5 Roman chicks,aged from 7 to 14 days,were used in this study.The cochlea dissdcted intactly were cultured in vitro.3HTdR was steadily added to the culture fluid 3 days after culture,and the cultures were terminated on the 3rd day and the 6th day after using 3HTdR,respectively and then the lagena epithelium at the distal tip of the BP was lighted and cut into sections to be observed.The results showed that there was low level proliferation of supporting cells in the lagena epithelium by mixing 3HTdR for 3 days and 6 days.The labelled supporting cells were mostly located in the basilar and the middle layers,a few cells moved to the surface of the epithelium.It was concluded that a continuous,low level proliferation of supporting cells existed in the macula of the lagena at the distal tip of neonatal chicks, suggeting that the factors of initiating and stimulating proliferation of the supporting cells might exist in the isolated cochlea epithelium.