川芎嗪逆转肿瘤抗药性与谷胱甘肽过氧化物酶的关系

The Correlationship between reversing effect of ligustrazine(LIG) on multidrug resistance and content of GSHPx in EAC/ADR cell lines

采用５，５′一二硫对硝基苯甲酸（ＤＴＮＢ）直接法对艾氏腹水癌（ＥＡＣ）及对阿霉素产生抗药性的ＥＡＣ耐药细胞（ＥＡＣ／ＡＤＲ）内谷胱甘肽过氧化物酶的活性进行测定。结果，ＥＡＣ／ＡＤＲ细胞内谷胱甘肽过氧化物酶的活性较ＥＡＣ细胞明显增高，分别依次为：（３７８±０１５）Ｕ及（０２９±００５）Ｕ；川芎嗪不同剂量与阿霉素合用，则能使ＥＡＣ／ＡＤＲ细胞部分恢复其敏感性，且谷胱甘肽过氧化物酶的活性也随之降低。结论：川芎嗪降低ＥＡＣ／ＡＤＲ细胞内谷胱甘肽过氧化物酶的活性，可能是其逆转肿瘤抗药性的机制之一。

The aim of this experiment was to study the correlationship between reversing effect of LIG on mutidrug resistance and contents of GSHPx in EAC/ADR cell lines.The results showed that the GSHPx activity in resistant cell line (EAC/ADR (3.78±0.15) U) was 13 fold higher than that in sensitive cell line (EAC (0.29±0.05)U);when LIG at doses of 10,20 and 30mg/kg was combined with ADR 1.0mg/kg,the sensitivity of EAC/ADR partially recovered and the GSHPx activity in EAC/ADR cell decreased.It is suggested that decreased GSHPx activity of EAC/ADR cell by LIG may be one of the mechanisms of reversing the drug resistance in EAC/ADR cell line.