显性负性ⅠκBα对乳腺癌细胞株核因子κB通路和运动迁移的影响

Effect of dominant negative ⅠκBα transfection on NF-κB pathway and cell motility in breast cancer

目的:探讨在高转移人乳腺癌细胞中,核因子κB活性对肿瘤生长及运动迁移能力的影响。方法:转染显性负性突变的ⅠκBα重组质粒入高转移乳腺癌细胞MDA-MB-231、MDA-MB-435,筛选并鉴定稳定转染的细胞株;凝胶迁移实验观察核因子κB活性,细胞生长曲线、平板集落形成试验及Millicell-PCF培养小室观察质粒转染对细胞生长和趋化运动的影响。结果:乳腺癌MDA-MB-231、MDA-MB-435细胞中存在高NF-κB组成型激活,稳定转染显性负性的ⅠκBα质粒后,细胞NF-κB活性下调,在不明显影响细胞生长、克隆形成的情况下,抑制高转移乳腺癌细胞株的运动能力。结论:在体外,抑制NF-κB通路,可明显抑制高转移乳腺癌细胞株的运动迁移能力。

AIM： To observe the effect on NF-κB pathway and cell motility in breast cancer cell lines after transfection of dominant negative IκBα plasmid. METHODS ： After stable transfection of mutant IκBα plasmid into highly metastatic breast cancer lines MDA - MB - 231 and MDA - MB -435, we detected NF-κB binding activity by EMSA, cell growth ability by cell growth curve, colony forming test, and cell motility by millicell - PCF chamber. RESULTS： Constitutive activities in MDA - MB -231 and MDA - MB -435 were observed. Stable transfection of a dominant negative IκBα resulted in downregulation of NF-κB binding activity, thus inhibited cell mobility without significant effect on cell growth. CONCLUSION： Cell migration ability is inhibited in highly invasive breast cancer cells by inhibition of NF-κB pathway in vitro.