固相萃取HPLC法研究六昧地黄方中马钱素和莫罗苷的血药浓度及配伍变化

Study on Loganin and Morronside Concentrations in Rat Serum Containing Liuwei Dihuang Formula by SPE-HPLC and the Variety of Different Combinations

目的建立测定六味地黄方中有效成分马钱素(loganin)和莫罗苷(morroniside)大鼠血清浓度的固相萃取HPLC法;研究大鼠灌胃给予六味地黄方后血清中马钱素和莫罗苷的浓度变化。方法血清样品经C_(18)固相萃取柱提取后,采用HPLC法测定给药前后大鼠药物血清中马钱素和莫罗苷的浓度,并对该方法进行方法学考察。结果血清中内源性物质不干扰样品中马钱素和莫罗苷的测定,马钱素和莫罗苷的线性关系良好,相关系数分别为0.999 8,0.999 8,方法回收率在95%～105%,提取回收率均大于75%,日内精密度和日间精密度均小于12%。结论该方法具有灵敏、快速的优点,可满足不同配伍六味地黄方有效成分在大鼠体内药代动力学研究的要求。不同配伍对药物血清中有效成分的吸收有一定影响。

Objective To establish a sensitive and specific liquid chromatography method for the determination of loganin and morroniside in rat serum containing Liuwei Dihuang Formula （LDF）. Methods Loganin and morroniside were extracted from the rat serum using SPE column, then separated on a Cls column. A HPLC method for determining serum concentration of mor- roniside and loganin in rats before and after administration LDF was established. Results A good linear calibration curve of logaMin and morroniside was obtained. The recovery of the method was in the range of 95 % - 105 %. The extracted recovery was more than 75 % , and within -day and intraday precisions were less than 12 %. Conclusion This method is sensitive, simple and rapid for monitoring of drug level in the study on pharmacokinetics and different combination of LDF.