肿瘤转移抑制基因KAI1在宫颈癌的表达及其与HPV感染的关系

Expression of Metastasis Suppressor Gene KAI1 in Cervical Carcinoma and Infections of HPV16 E6, E7 and HPV18 E6/E7

目的探讨肿瘤转移抑制基因KAI1在宫颈癌中的蛋白表达以及人乳头瘤病毒(HPV)16E6、E7和HPV18E6/E7感染对其表达的影响。方法采用免疫组化SP法检测70例浸润性宫颈癌、15例原位癌、20例正常宫颈组织中KAI1蛋白的表达,采用PCR扩增-琼脂糖凝胶电泳法检测浸润性宫颈癌组织中HPV16E6、E7和HPV18E6/E7的DNA状况。结果宫颈浸润癌及原位癌组织中的KAI1蛋白表达与正常宫颈上皮中的表达相比明显下调(P<0.05),原位癌与浸润癌中的表达差异无统计学意义;HPV16E6、E7和HPV18E6/E7在浸润性宫颈癌中的阳性率分别为67.1%、54.3%和12.9%,HPV16E6、E7和HPV18E6/E7感染与宫颈癌中的KAI1蛋白表达无相关性。结论肿瘤转移抑制基因KAI1在宫颈癌中呈下调表达,HPV16E6、E7和HPV18E6/E7感染不影响其表达。

Objective To investigate the expression of metastasis suppressor gene KAI1 in cervical carcinoma and the impact of human papillomavirus16 E6, E7, 18 E6/E7 infection on the expression of KAI1. Methods The expressions of KAI1 protein in the formalin-fixed, paraffin-embedded specimens of 20 normal cervical epthelium, 15 cervical in situ carcinoma and 70 primary invasive cervical carcinoma were detected by immunohistochemistry SP. Polymerase chain reaction （PCR） tests were also undertaken to detect the HPV16 E6, E7 and HPV18 E6/E7 DNA. Results The expression of KAI1 protein was down-regulated in the invasive carcinoma and in situ carcinoma compared with the controls （P^0.05）. No significant difference was found in the expression of KAI1 protein between invasive carcinoma and in situ carcinoma. The infections of HPV16 E6, E7 and HPV18 E6/E7 were found in 67.1%, 54.3% and 12.9% of the invasive carcinoma, respectively. However, there was no correlation between the expression of KAI1 and the infections of HPV16 E6, E7 and HPV18 E6/E7. Conclusion The expression of KAI1 protein is down-regulated in cervical carcinoma, which is not associated with the infection of HPV16 E6, E7 and 18 E6/E7.