窄谱中波紫外线和/或阿维A对角质形成细胞异维A酸受体α mRNA表达的影响

Alteration of Retinoid X Receptor α mRNA Level after Acitretin and/or Narrow-band UVB Treatment in Normal Human Keratinocytes

目的研究阿维A和/或窄谱中波紫外线（NB-UVB）对培养的正常人角质形成细胞异维A酸受体α（RXRα）mRNA表达的影响。方法用10^-7 - 10^-6mol/L阿维A和/或50-100mJ/cm^2NB-UVB处理正常人角质形成细胞后,继续培养12h,用RT-PCR和实时荧光定量RT-PCR法检测角质形成细胞RXRα mRNA表达的改变。结果50-100mJ/cm^2NB-UVB单独照射角质形成细胞后,可抑制RXRα mRNA的表达;10^-7 -10^-6mol/L阿维A单独处理后,RXRα mRNA的表达无明显降低,二者联合作用时,对RXRα mRNA的表达抑制作用更强。结论NB-UVB单独作用可明显抑制RXRα mRNA的表达,阿维A单独处理对RXRα mRNA的表达无明显影响,但二者联合作用时对RXRα mRNA的表达存在协同抑制作用。

Objective To investigate the alteration of retinoid X receptor α（RXRα） mRNA level in normal human keratinocytes after acitretin and/or NB-UVB irradiation treatment. Methods After a 12-hour incubation with 10^-7 - 10^-6 mol/L acitretin and/or following 50-100 mJ/cm^2 NB-UVB irradiation in normal human keratinocytes, RXRa mRNA expression was examined by reverse transcription polymerase chain reaction （RT- PCR） and real-time quantitative RT-PCR. Results The expression of RXRa mRNA was obviously decreased by NB-UVB irradiation, but not by acitretion single treatment. When combining acitretin treatment with NB-UVB irradiation, greater decreased RXRa mRNA expression was observed than that of single treatment. Conclusion Narrow-band UVB irradiation treatment can decrease RXRa mRNA expression, but not acitretin single treatment. Combining treatment with both can produce synergistic inhibition effects.