五倍子提取物对大鼠龋病的抑制作用

Inhibitive Effect of Extracts of Galla Chinesis on Caries Development in Rats

目的利用大鼠龋模型评价五倍子提取物——五倍子E（Galla Chinesisextract,GCE,总鞣质）和五倍子B（Galla Chinesisextract-B,GCE-B,没食子酸和没食子酸甲酯）的抑龋作用。方法用S.sobrinus感染SPF-SD大鼠口腔,建立龋病模型。以GCE、GCE-B为实验组,氟化钠（NaF）、洗必泰（CHX）为阳性对照组,去离子水为阴性对照组,每日药物处理大鼠牙齿2次,连续5周。处死大鼠,记录Keyes龋齿记分。结果对光滑面E级龋损,与去离子水组相比,GCE组、GCE-B组、CHX组、NaF组都显示了较强的抑龋作用（抑制率43%-61%,P〈0.05）;NaF组、GCE组和CHX组均未检出光滑面Ds级龋损,GCE-B组有检出,低于阴性对照组,但差异无统计学意义。对窝沟E级龋损,与去离子水组相比,GCE组、GCE-B组、CHX组、NaF组记分均有明显降低（16%-41%,P〈0.05）,并显著降低了Ds级（21%-47%）、Dm级（21%-65%）龋损（P〈0.05）;对Dx级龋损,GCE组、CHX组、NaF组均未检出,GCE-B组有所降低（60%,P〈0.05）。结论五倍子提取物具有明确的抑龋作用,GCE优于GCE-B。

Objective To evaluate the inhibitive effect of Galla Chinesis extract （GCE） and GCE-B on dental caries formation and plaque microbiology in rats. Methods SPF-SD rats were infected with S. sobrinus to establish the caries rat model. The rats＇ teeth were treated with GCE and GCE-B topically twice a day for 5 weeks, or with NaF and CHX as positive controls, and deionized water as negative controls. The rats were then killed with CO2 asphyxiation. The dental caries of the rats were evaluated with Larson＇s modification of Keyes＇ system. Results The treatments with GCE and GCE-B significantly reduced the incidence of smooth-surface caries compared with the negative controls（43%-61% reduction, P〈0.05）. No caries of Ds level was detected in the rats treated with NaF, GCE, or CHX. The treatment with GCE-B reduced the severity of smooth-surface caries compared with the negative controls, but with no statistical significance （P〉0.05）. The incidence of sulcal caries were reduced significantly by all treatments （16%-41% reductions, P〈0.05） compared with the negative controls. The sulcal caries severity scores were significantly lower in the rats treated with NaF, GCE, CHX and GCE-B （Ds level： 21%-47% reductions, Dm level： 21%-65% reductions） than in the negative controls （P〈0. 05）. The reduction of Dx score by GCE-B （60% reduction） was significant （P〈0.05）. No caries of Dx level was detected in the rats treated with NaF, GCE and CHX. Conclusion GCE and GCE-B have anticaries effect in vivo, and GCE shows stronger effect than GCE-B.