HPLC法测定红霉素软膏含量

Discussion On The Determination Of Erythromycin Ointment By HPLC

建立高效液相色谱法测定红霉素软膏的含量。采用Sinochrom ODS-BP C18为分析柱。流动相：0．1mol·L^-1磷酸二氢铵溶液（三乙胺调节pH6．5）-乙腈（70：30）；流速：1．0mL·min^-1；检测波长：215nm。进样量：20μL。在进样量2．5～80．0μg的范围内，进样量与峰面积线性关系良好（r=0．9999）；平均回收率98．94％；RSD=0．4％。该方法简便快速，重现性好，可作为红霉素软膏的含量测定方法。

A HPLC method was used for determination of Erythromycin Ointment, A Sinochrom ODSBP C18 column was used with 0. 1mol · L1 ammonium dihydrogen phosphate （adjust the pH to 6.5 with triethylamine）-acetonitrile （70 ： 30） as mobile phase. Detective wavelenth was at 215nm with flow rate of 1.0mL · min^-1. The good linearity range was within 2.5-80.0μg（r=0. 9999）, RSD=0.31%. The method is simple and rapidness, has good repeatability, it can be used in the quality control of Erythromycin Ointment.