HPLC法测定小儿退热口服液中丹皮酚的含量

Determination of Paeonol in Xiaoer Tuire Oral Solution by HPLC

建立小儿退热口服液中丹皮酚的含量测定方法。采用高效液相色谱法。色谱柱为Kromasil C18柱，流动相为甲醇-水（53：47），流速为0．9mL·min^-1，检测波长为274nm，柱温为30℃。丹皮酚进样量在0．04124-0．4124μg范围内线性关系良好，r=0．9999。平均回收率为99．20％（n=9），RSD为0．5％。本法简便、准确，可用于产品的质量控制。

To establish a method for determination of paeonol in Xiaoer Tuire Oral Solution by HPLC. HPLC was use, using a Kromasil C18 column, and methanol and water （53 ： 47） as the mobile phases the detecting wavelength was 274nm. The calibration curve of paeonol was linear within the range of 0. 04124-0. 4124μg, the average recovery was 99. 20% （n=9）. The method is simple and accurate. It can be used for the quality control method of Xiaoer Tuire Oral Solution.