HIV感染者中结核杆菌ESAT-6蛋白特异性T细胞反应的检测

Specific T-cell responses to Mycobacterium tuberculosis protein ESAT-6 in Chinese HIV positive individuals

目的检测结核杆菌抗原早期分泌抗原靶分子（ESAT-6）融合蛋白其在人类免疫缺陷病毒感染患者中特异性T细胞反应，从而为艾滋病合并结核杆菌感染的诊断方法的建立提供理论依据。方法利用重组表达结核杆菌ESAT-6蛋白做为抗原，通过酶联免疫斑点技术（ELISPOT）检测HIV感染者中特异性T细胞反应，并以正常健康者、无HIV感染的结核患者作为对照。结果ESAT-6重组蛋白对结核患者外周血单核细胞ELISPOT方法检测证实其具有良好的抗原性，斑点形成单位数（SFU／10^6PBMC）在结核组和HIV合并结核感染组中显著升高，并与无合并结核感染临床表现的HIV组以及健康对照组间均存在明显的差别。结论重组结核杆菌ESAT-6蛋白可以作为抗原通过ELISPOT方法检测HIV感染者中结核杆菌合并感染。

Objective To detect TB specific T cell responses by using the recombinant ESAT-6 protein as stimulus in Chinese HIV infected patients. Methods ELISPOT-IFN-γ assay by using the recombinant ESAT-6 protein as stimulus to detect specific T cell responses in HIV（ ＋ ） patients with or without clinical manifestation of TB diseases. Results Recombinant ESAT-6 protein specific T cell responses show significant high frequencies in both of TB patients with or without HIV infection than that in the healthy control and HIV （ ＋ ） group without clinical TB diseases. Conclusions The ELISPOT-IFN-γ assay by using recombinant ESAT-6 protein as stimulus could be used in diagnoses of TB infection in Chinese HIV infected patients.