球形多孔支架材料体内血管化的量化分析

Quantification of vascularization within spherical porous β-TCP scaffold in vivo

[目的]研究球形多孔β-磷酸三钙(β-TCP)支架植入体内后的血管形成情况。[方法]选择成年新西兰兔30只,随机分为5组,每只兔植入一个球形β-TCP材料(直径2cm,孔径500～600μm,内连接径110～120μm),于术后1、2、4、8、12周取材进行组织学观察、定量分析血管长入情况。[结果]术后1周未见新生血管。术后2周,支架外周见细小幼稚的血管。4周时,为血管化的第一个高峰,与2周比血管数量增加(P<0.05)。8周时,血管数及直径增加,但无统计学意义(P>0.05)。12周时,为第2次血管化高峰,见粗大成熟的血管,与其他时间比血管直径增大(P<0.05)。[结论]血管化分阶段进行,即早期数量的增加和后期质量的提高。材料的结构和降解特性影响血管化。

[ Objective] To explore the vascularization within spherical porous I3-TCP scaffolds in vivo. [ Method ] Thirty aduh rabbits were selected and divided into 5 groups randomly. Spherical porous [3-TCP scaffolds（ the diameter was 2 cm, the pore size was 500 ～600μm,the interconnection size was 110～120 μm） were harvested at 1,2,4,8 and 12 weeks after embedded into muscle-fascia lumbodorsalis pouches in each rabbit separately,in order to observe the vascularization by means of morphological and quantificational analysis. [ Result ] No vessel was detected at one week after surgery, and only a few immature vascular buds could be seen at the 2nd week. The first vascularization peak could be observed at 4 weeks ,characterized by the growth of quantity of new blood vessels （P 〈 0.05 ）. At 8 weeks ,vascnlature reform happened due to relative insufficient supply of oxygen and nutrition, showing the enlargement of new vessels diameters. At 12 weeks,the 2nd vascularization peak was shown that the diameter of new vessels increased（P 〈0.05）and mature vessels had thick walls and little branches, while the numbers of vessels remained stable（P 〉0.05 ）. [ Conclusion] The process of vascularization comprises two phases, the quantity increasing in the early stage and the quality enhancing in the later stage, The special architecture and biodegradation characters of scaffolds could influence the vascularization.