摘要
本研究探讨了一组具有分解纤维素和农药林丹双重功能的复合菌系NSC-7的培养特性和稳定性。NSC-7在14d的培养过程中,使稻秆分解73.6%;用GC-MS测定结果发酵液中检测到10种化合物成分,其中峰值较大的依次为乙酸、甘油、丁酸、丙酸;NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性;经90℃高温处理30min后,仍能够保持分解能力,105℃处理30min后转接2次就能恢复分解能力,显示出很高的保存稳定性和热稳定性。利用DGGE分析多次继代接种过程的培养物结果条带基本没有变化,表明NSC-7的菌种组成稳定。
Abstract： The cultural characteristics and stability of the bacteria community NSC-7, which was capable of degrading cellulose and lindane with high efficiency was discussed in this study. NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatog- raphy mass spectrometry （GC-MS）, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave. It suggested that NSC-7 had efficient conservation stability and thermo-stability.DGGE analysis showed that the composition of NSC-7 remained stable after subcultured for several times.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第5期725-730,共6页
Microbiology China
基金
国家“十一五”支撑计划(No.2006BAD25B04)
国家自然科学基金(No.30571088)
东北林业大学青年基金(No.07035)
