阿托伐他汀对血管内皮细胞增殖过程中FKBP12基因表达的影响

Effects of atorvastatin on gene expression of FKBP12 in vascular endothelial cell proliferation

目的探讨不同浓度阿托伐他汀对血管内皮细胞（VEC）增殖过程中FK506结合蛋白12（FKBP12）表达的影响。方法培养大鼠VEC并传代，检测Ⅷ因子相关抗原。观察其不同浓度阿托伐他汀作用下的VEC增殖情况和不同时期FKBP12 mRNA水平的表达。结果阿托伐他汀抑制大鼠血管内皮细胞的增殖过程，抑制血管内皮细胞增殖过程中FKBP12的表达，该作用呈时间依赖性和剂量依赖性；在阿托伐他汀作用下，大鼠血管内皮细胞数随浓度的增加和作用时间的延长呈相对减少趋势。结论阿托伐他汀抑制血管内皮细胞的增殖过程，抑制血管内皮细胞增殖过程中FKBP12的表达，且呈时间-剂量依赖性。

Objective To evaluate the effect of atorvastatin on gene expression of FK506 binding protein 12（FKBP12） in vascular endothelial cell （VEC）proliferation. Methods Rat vascular endothelial cells were cultured with enzyme digesting methods, and were identified by measuring their growth rate with invertmicroscope, by using immuocytochemical methods with factor Ⅷ antibody. Then the cells were treated with atorvastatin in different concentration. The proliferation of rat vascular endothelial cell was observed under an invert microscope. The expression of FKBP12 mRNA was detecteb by semi - quantitative RT - PCR in different culture periods. Results Atorvastatin markedly restrained the expressions of FKBP12 mRNA in VES. The effect appeared dose dependence and time dependence. With the effect of atorvastatin, the downtrend of VEC count was evident along with the increased concentration the prolonged time. Conclusion Atorvastatin dose -dependently and time - dependently restrained the proliferation of rat VEC. As a result, the expression of FKBP12 mRNA is decreased too.