血浆血管内皮生长因子和尿微量白蛋白及β2-微球蛋白与过敏性紫癜患儿早期肾损伤的相关性研究

Plasma vascular endothelial growth factor, urine microalbumin and β2-microglobulin in children with early renal involvement of henoch - schonlein purpura

目的探讨血浆血管内皮生长因子（VEGF）、尿微量白蛋白（MA）及β2-微球蛋白（β2-MG）与过敏性癜（HSP）及过敏性紫癜肾炎（HSPN）发病关系；探寻HSP早期肾损伤敏感的实验室指标。方法实验分组为：过敏性紫癜组，根据送标本时临床诊断标准分组为：皮肤型组、混合型组、HSPN型组；健康对照组；治疗后组。本实验测定血浆VEGF采用ABC—ELISA法，测定尿MA及β2-MG采用放射免疫分析法。所有统计资料采用SPSS12．0统计分析软件包进行分析，以P〈0．05为差异有统计学意义。结果HSP早期各临床分型比较发现HSPN型组血浆VEGF、尿MA及β2-MG水平分别高于其他临床型，差异有统计学意义（P〈0．05）；治疗组高于对照组（P〈0．05），差异有统计学意义。血浆VEGF、尿MA及β2-MG阳性率分别与其他指标阳性率相比较差异有统计学意义（P〈0．05）。结论HSP早期患儿血浆VEGF、尿MA及β2-MG水平均明显增高，伴有肾损伤者升高尤为明显，恢复期有所降低但仍处于较高水平。本研究结果表明血浆VEGF、尿MA及β2-MG是早期HSP并发肾损伤的敏感指标。

Objective To detect and explore the changes of plasma vascular endothelial growth factor （VEGF） urine microalbumin（MA） and β2 - Microglobulin（ β2 - MG） in children with henoch - schonlein purpura （HSP）. Methods Children involved in the experiments were divided into three groups： HSP group： HSP were divided into pure purpura group, mixed pattern group and HSPN group; normal control group; recovery stage group. The experiment was double - antibody sandwich ABC - ELISA detected VEGF, detected MA and β2 - MG by radioimmunoassay. Results HSP comparison of clinical classification found that the plasma VEGF, urine MA and β2 - MG concentration at acute period of renal involvement group compared with others groups show statistical significance （ P 〈 0. 05 ）. After - treated groups was higher than that in control group（ P 〈 0. 05）. In the early period of 73 HSP children, high β2 -MG 49 cases （67.12%） ,more VEGF 51 cases （69.86%）, MA 53 cases （72.60%）highest. VEGF than other index（ P 〈0.01 ）. Conclusion The average levels of plasma VEGF, urine MA and β2 - MG are increased significantly particularly with early renal damage in HSP patients. Plasma VEGF, urine MA and β2 - MG are sensitive markers of renal damage in early stage of HSP patients.