婴幼儿仙台病毒天津株感染的IgM抗体检测及意义

Detection and Significance of Sendai Virus Specific IgM Antibody in Infant

目的:通过对婴幼儿仙台病毒天津株感染的IgM抗体检测,了解人兽共患病原体——仙台病毒对婴幼儿急性下呼吸道的致病情况。方法:观察组为2006年冬季天津市儿童医院0～7岁首诊呼吸道感染患儿83例血清标本,通过间接免疫荧光检测法(IFA)排除了7种常见呼吸道病毒的感染,用自建的酶联免疫吸附法(ELISA)对仙台病毒的IgM抗体进行检测,同时设20例正常儿童血清标本为阴性对照组。结果:观察组16例为阳性,阳性率19.28%。与2004年同期数据44.59%(33/74)相比,本组阳性率有所降低,差异有统计学意义(P<0.01)。IgM抗体检测阳性率1岁内最高,1～3岁和3～7岁组阳性率基本接近,但3组之间差异无统计学意义(P>0.05)。结论:仙台病毒在儿科急性呼吸道感染的病原体中占有一定的比例,不可忽视。应对其感染状况进行深入研究。

Objective： To investigate the morbidity of infant with acute lower respiratory, tract infection through the detection of Sendai Virus （SeV）,Tianjin strain,specific IgM antibody. Methods： Eighty three sera were taken from the children of 0-7 years old who first visited Tianjin Children＇s Hospital due to respiratory, tract infections. The 7 common viral agents causing respiratory, tract infections were excluded by indirect immunofluorescence assay. The SeV specific IgM was detected by ELISA comparing to 20 normal sera as control group. Results： The IgM positive was detected in 16 sera of treated group （19.28%）. This positive rate was significantly decreased compared with that of the past year （P 〈 0.01 ）. The highest detected positive rate was found in 1 year old group. The positive rate was nearly the same between 1-3 and 3-7 age groups. There were no significant differences among the above three age groups. Conclusion： The SeV is supposed to be one of most important pathogens which results in acute respiratory, tract infections in children. Therefore,further investigation should be carried out.