血管内皮抑素与顺铂对Calu-6肺癌细胞联合作用的观察

Synergistic Effect of Endostatin and Cisplatin on Calu-6 Lung Cancer Cells

目的:研究血管内皮抑素对人肺癌细胞的增殖抑制作用及机制,并观察其与顺铂(DDP)的联合作用。方法:经血管内皮抑素及DDP干预后,以MTT法检测其对肺癌细胞Calu-6的抑制、流式细胞术检测细胞凋亡、ELISA测定Bcl-2/Bax、sFas/sFasL的表达。结果:(1)血管内皮抑素具有抑制Calu-6细胞增殖的作用(P<0.05)并呈现时间依赖性、诱导Calu-6细胞凋亡,下调Bcl-2表达,但对Bax的表达无明显影响(P>0.05),未检测到sFas/sFasL的表达。(2)血管内皮抑素与DDP联合用药方案中,同时给药较单药DDP诱导凋亡及下调Bcl-2表达明显,序贯给药与DDP间无明显差异(P>0.05)。(3)2种血管内皮抑素(恩度、内皮抑素)的效应差异无统计学意义(P>0.05)。结论:(1)血管内皮抑素可抑制Calu-6细胞生长、诱导其凋亡,其机制与下调Bcl-2表达有关。(2)血管内皮抑素与DDP间有增效作用,联合用药方案中以同时给药效果最著。(3)各血管内皮抑素的作用机制相似。

Objective： To investigate the inhibitive effect of endostatin and synergistic effect of endostatin with cisplatin （DDP） on human lung cancer cells and their mechanisms. Methods： The inhibition of Calu-6 cell proliferation was measured by MTT assay after treatment with endostatin and/or DDP in lung cancer cells. The cell apoptosis was measured by flow cytometry （FCM）. The expressions of Bcl-2/Bax and sFas/sFasL were detected by ELISA. Results： （ 1 ）Compared with control group, endostatin significandy inhibited Calu-6 cell proliferation （P 〈 0.05） with time-dependence and induced cell apoptosis. Also the expression of Bcl-2 was down-regulated by endostatin whereas no obvious effect on Bax（P 〉 0.05 ）. The expression of sFas/sFasL was not detected. （2）The down-regulation of Bc1-2 expression and cell apoptosis were significance in combinative administration of endostatin and DDP compared with those of cisplatin alone. Successive administration represented no obvious effect than that of DDP （P 〉 0.05 ）. （3）There were no significant differences among three different kinds of endostatins （P 〉 0.05 ）. Conclusion： Endostatin can inhibit growth of Calu-6 cells and induce its apoptosis, which related to the down-regulation of Bcl-2 expression. There is a synergistic effect between endostatin and DDP. There are similar effects among different kinds of endostatin.