SPE-HPLC法测定啤酒中的微量反-2-壬烯醛

Determination of trans-2-nonenal in beer by SPE-HPLC

采用固相萃取-高效液相色谱法对啤酒中的微量反-2-壬烯醛进行检测。啤酒样品经水蒸汽蒸馏、Supelclean LC-18固相萃取小柱净化后，采用Nucleosil 100—5C18色谱柱（250mm×4．0mm，5μm）分离，流动相乙腈：水：55：45（V／V），流速为1mL／min，检测波长226nm。在10～150μg／L范围内，峰面积与反-2-壬烯醛浓度呈良好的线性关系（r=0．9998），精密度（RSD）小于5％，样品浓缩250倍后检测限为0．25μg／L，平均回收率在80．4％-109．8％之间。

An analytical method was developed to determine trans-2-nonenal in beer involving steam distillation of beer followed by a clean up step using solid phase extraction and determination of trans-2-nonenal by HPLC.A column Nucleosil 100-5 C18（250mm ×4.0mm,5μm） was used.A water-acetonitrile （45：55, V/V） mixture was used as mobile phase, with a flow rate of 1mL/min. The detection wavelength was set as 226nm. There was a good linear correlation between peak areas and the concentration of trans-2-nonenal with a range of 10-150μg/L. The RSD of the method was less than 5%. The average recoveries were 80.4%- 109.8% and the detection limit of 0.25μg/L was obtained after a 250-fold of concentration of beer samples.