RYK在小鼠皮质脊髓束神经通路发育过程中的表达变化

RYK expression changes during the developmental projection of corticospinal tract pathway in mice

目的观察RYK在皮质脊髓束发育投射通路中的表达情况，为进一步研究RYK在其中的功能提供重要线索。方法通过原位杂交和免疫荧光组织化学法，分别检测了E14．5、E16、E18．5和出生后P3、P5、P7等不同时间点RYK在小鼠大脑运动皮层和皮质脊髓束神经通路中的表达情况。结果RYK的表达出现在胚胎E18．5d运动皮层神经元中，到P0时逐渐增强并表达在脑内投射的皮质脊髓束神经通路，从P0～P5，RYK持续表达在皮质脊髓束在脑内和脊髓的整个神经通路上，而后逐渐减弱。结论RYK的表达与皮质脊髓束神经通路在脊髓中的发育投射有密切关系。

Objective To study the expression pattern of RYK in the pathway of developmental corticospinal tract projection and provide hints for further study on RYK＇ s function involved in this processes. Methods By In situ and immuno-fluorescence histochemistry, we examined the RYK expression pattern in the developmental murine motor cortex and the projection of corticospinal tract at various time points of E14.5, E16 ,E18. 5 and postnatal P3, P5 ,P7. Results The motor cortex the RYK expression started at E18.5 stage and enhanced gradually at P0 stage. Moreover from PO to P5 stage the RYK expression also appeared in the pathway of developmental corticospinal tract and decreased gradually after that. Conclusion RYK＇ s expression is closely related to the corticospinal tract projection during its development stage.