摘要
从海南不同地区采集芒果炭疽菌(Colletotrichum gloesporioides Penz.)进行多菌灵抗性检测。敏感性测定结果表明:7个地区共23个菌株中,有2个地区的5个菌株表现出高度抗药性,在含1200μg/mL多菌灵的PDA培养基上菌丝生长良好,EC50值为520.95～746.26μg/mL。抗性菌株对苯并咪唑类其它杀菌剂如噻菌灵和甲基托布津测定表明,苯并咪唑类杀菌剂之间存在正交互抗药性;对咪鲜胺则表现敏感。连续无毒培养8代后,5个菌株仍可在1200μg/mL多菌灵的培养基上生长,抗性水平保持不变。通过致病力及孢子萌发对抗药菌株的适合度进行测定,抗药性菌株的致病能力和孢子萌发率无明显变化,表现很高的适应能力,说明这类菌株在田间有很强的竞争力。
Twenty three isolates of Colletotrichum gloesporioides, a causal agent of mango anthracnose, were collected from 7 regions in Hainan Province during the period from 2003 to 2005, and their resistance to carbendazim was determined. The results showed that 5 isolates collected from 2 regions were resistant to carbendazim at a high level. These 5 isolates grew well at 1 200 μ/ml of carbendazim, and their EC5o values of inhibiting mycelium growth were 520.95 μ/mL ～746.26 μ/mL. In cross resistance test, these isolates showed positive cross resistance to benzimidazole fungicides such as thiabendazole, thiophanate-methyl, but sensitive to Prochloraz. The 5 isolates grew well at 1 200 μ/mL of carbendazim after 8 times of subcultures on the fungicide-free PDA, and kept its resistance to carbendazim. Analysis of their pathogenicity and sporulation showed no evident change, indicating their strong competition in the field.
出处
《热带作物学报》
CSCD
2008年第1期73-77,共5页
Chinese Journal of Tropical Crops
基金
农业微生物菌种资源整理、整合及共享试点项目(2004DK30560-8)
农业结构调整重大技术研究专项项目(2003-10-02A)
关键词
芒果炭疽菌
多菌灵
抗药性
海南
mango
Colletotrichum gloesporioides
Carbendazim resistance
Hainan
