寻常型银屑病皮损中Smad2、Smad3 mRNA的表达降低

Low expression of Smad2 and Smad3 mRNA in lesions of psoriasis vulgaris

目的:检测寻常型银屑病皮损中Smad2和Smad3的mRNA表达水平。方法:采用反转录-实时定量聚合酶链式反应技术对寻常型银屑病皮损与正常对照皮肤中Smad2和Smad3 mRNA的表达水平进行分析。结果:与正常对照皮肤相比,寻常型银屑病皮损Smad2和Smad3 mRNA的表达水平均显著降低。结论:寻常型银屑病皮损中Smad2和Smad3表达下调,可能通过干扰TGF-β信号向下游转导,有助于寻常型银屑病皮损角质形成细胞过度增殖状态的形成。

Objective： To investigate the expression of Smad2 and Smad3 mRNA in psoriatic lesion. Method： Reverse transcriptase-quantitative real-time polymerase chain reaction （RT-quantitative Real- Time PCR） was used to measure the expression levels of Smad2 and Smad3 mRNA in psoriatic lesions compared with those in normal control skin specimens, Results： The expression of Smad2 and Smad3 mRNA was significantly down- regulated in psoriatic lesions compared with those in normal control skin. Conclusion： Down - regulated expressions of Smad2 and Smad3 in psoriatic epidermis may contribute to the formation of hyperplastic epidermis in psoriatic lesions via blocking TGF-β signaling.