摘要
The pigments in Laminaria japonica was extracted with six organic solvents and analyzed in spectroscopy analysis. The extractions conditions were screened by an orthogonal test and the quantity of extracted pigments was determined spectroscopically. The results show that: (1) among the six organic solvents, acetone was the most effective one for the extraction; (2) the optimum extraction conditions were as follows: the ratio of S/M (solvent volume/material weight) was 30 ml/g; fresh seaweed was extracted 2 times in 2h; (3) the average total content of pigments was 1.85 mg/g (calculated with dry L. japonica).
The pigments in Laminariajaponica was extracted with six organic solvents and analyzed in spectroscopy analysis. The extractions conditions were screened by an orthogonal test and the quantity of extracted pigments was determined spectroscopically. The results show that： （1） among the six organic solvents, acetone was the most effective one for the extraction; （2） the optimum extraction conditions were as follows： the ratio of S/M （solvent volume/ material weight） was 30 ml/g; fresh seaweed was extracted 2 times in 2 h; （3） the average total content of pigments was 1.85 mg/g （calculated with dry L. japonica）.
基金
the Technical Program of Jiangsu Province (BE 2006383)
the Talent Program of Chinese Academy of Sciences (Dongbei Zhi Chun)
关键词
昆布属植物
色素提取
测试方法
海洋化学
fresh Laminaria japonica
total pigment extraction
orthogonal test
