摘要
根据鸽生长激素受体(GHR)基因CDS序列设计引物进行PCR扩增,克隆了鸽GHR基因内含子9序列,通过Gen-Bank中Blastn比对表明,与鸡内含子9(AF372659)同源性为70.01%。利用PCR-SSCP技术和DNA测序方法检测GHR基因内含子9、外显子10和3’-UTR的单核苷酸多态性。本试验共检测出3个多态位点,经最小二乘法分析,多态位点与两群体鸽生长性状及体组成均不相关。
The sequence of GHR gene intron 9 were cloned by one pair of primers that was designed according to the CDS sequence of GHR gene. The homology of this sequence with chicken was 70.01% through Blastn of GenBank. SNPs of GHR gene intron 9, exon 10 and 3＇-UTR were identified and genotyped by PCR-SSCP analysis and DNA sequencing in two pigeon populations. 3 SNPs were detected in the research and analyzed by the least square analysis, the result showed that the genotypes of mutation had no significant association with growth traits and body composition.
出处
《中国畜牧兽医》
CAS
2008年第5期47-51,共5页
China Animal Husbandry & Veterinary Medicine
