308nm准分子激光对角质形成细胞分泌细胞因子的影响

308nm Excimer Laser Irradiation Up-regulates Keratinocyte-derived Cytokine

目的探讨308nm准分子激光对人角质形成细胞(HKC)增殖及分泌细胞因子的影响。方法CCK-8法检测不同剂量准分子激光照射后12h及24h对HKC增殖的影响;ELISA法检测细胞培养上清液中干细胞生长因子(SCF)、集落细胞生长因子(GM-CSF)、成纤维细胞生长因子(bFGF)的浓度;DNA Ladder法检测准分子激光照射后HKC晚期凋亡情况。结果细胞活力与照射剂量呈负相关,照射后12h的细胞活力与24h相比差异有显著性(P=0.001);308nm准分子激光剂量在100～300mJ/cm2时,HKC分泌SCF,GM-CSF,bFGF的增加呈剂量依赖性,与对照组(0mJ/cm2)比较差异有显著性(P<0.001),6h后分泌的SCF,GM-CSF,bFGF开始增加,24h后达到峰值。准分子激光照射可诱导HKC出现晚期凋亡。结论308nm准分子激光可能通过诱导角质形成细胞分泌SCF,GM-CSF,bFGF等促进黑素细胞增殖分化的细胞因子,使白癜风皮损复色。

Objectives The present study was designed to investigated whether 308 nm exeimer laser affects keratinocyte - decived factors cytokine by keratinocyte cultured. Methods Cultured keratinocyte was treated with various dosage of 308 nm excimer laser, The effects proliferation of keratinocyte by excimer laser were evaluated using CCK-8, ELISA was applied to evaluate the production of SCF,GM-CSF,bFGF in supernatant of HKC treated with excimer laser, Apoptosis was induced in HKC exposed to excimer laser. Results Proliferation of HKC was significantly reduced dosage-dependently with excimer laser. SCF, GM-CSF, bFGF secretion by HKC increased with increasing 308nm excimer laser（ ranged 100 -300 mJ/cm^2, which showed statistically difference to the control group（0 mJ/cm^2）. Secretion of those factor started after 6h of radiation, Exposure to excimer laser can induced apoptosis in HKC as assessed by DNA Ladder. Conclutions Our study provides in vitro demonstrating that HKC creates a favourable milieu through the secretion of some cytokine for melanocyte growth and migration.