线栓法制作大鼠局灶性脑缺血再灌注模型的改进与探讨

The improvement and discussion of focal cerebral ischemia-reperfusion injury model with patching method in rats

目的提供一种比较简易的大鼠局灶性脑缺血/再灌注模型制备方法。方法应用SD雄性大鼠,线栓法制作局灶性脑缺血再灌注模型,选择在颈总动脉(CCA)分叉处切口,使线栓从CCA顺行进入颈内动脉(ICA)。造模后直接将栓线缝合在皮内,再灌注时拔出一定长度拴线,使栓线回至CCA即可。通过神经功能检测,红四氮唑(TTC)染色和病理学检查等方法评价该模型的可靠性。结果大鼠手术后神经功能缺损明显,TTC染色清楚显示了梗死病灶,病理学检查显示典型病理改变。结论该改良方法术式简便,缺血效果可靠,可以用于脑缺血再灌注的研究。

Objective To establish a simple rat model of focal cerebral ischemia reperfusion injury. Methods The model of focal cerebral ischemia, reperfusion injury was made with patching method in SD rats, we cut open the common carotid artery（CCA） in the furcation and sent the patching enter into the internal jugular vein （IGV）along the CCA. After the model builded,the patching was sutured in skin. When the rat brain was perfused again, the patching was drawed out from IGV to CCA. The models were evaluated with neurological monitoring, TTC staining and pathological examination. Results The rats developed typical symptoms of neurological impairment. TTC staining showed the pale at the cerebral infarc. Typical pathological changes were presented by pathological examination. Conclusions The improving method was so simple and reliant that it could be used in the study of cerebral ischemia- reperfusion.