摘要
Objective: To investigate the effect of decreasing activity of NF-κB on proliferation of A549 cell line and the pos-sible molecular mechanism. Methods: The recombinant plasmid PCDNA3.1(+)/IκBα expressing IκBα was constructed. The recombinant plasmid was then transfected to A549 cell. The activity of NF-κB, cell proliferation, and cyclin D1 expression were observed. Results: Our results showed that transfecting PCDNA3.1(+)/IκBα inhibited activity of NF-κB in A549 cells, and decreasing activity of NF-κB inhibited proliferation of A549 cells. Decreasing activity of NF-κB was accompanied with down-regulation of cyclin D1 expression. Conclusion: Decreasing activity of NF-κB inhibited proliferation of A549 cells, and the molecular mechanism of the inhibition effect may be down-regulation of cyclin D1 expression.
Objective： To investigate the effect of decreasing activity of NF-κB on proliferation of A549 cell line and the possible molecular mechanism. Methods： The recombinant plasmid PCDNA3.1（＋）/IκBα expressing IκBα was constructed. The recombinant plasmid was then transfected to A549 cell. The activity of NF-κB, cell proliferation, and cyclin D1 expression were observed. Results： Our results showed that transfecting PCDNA3.1（＋）/IκBα inhibited activity of NF-κB in A549 cells, and decreasing activity of NF-κB inhibited proliferation of A549 cells. Decreasing activity of NF-κB was accompanied with down-regulation of cyclin D1 expression. Conclusion： Decreasing activity of NF-κB inhibited proliferation of A549 cells, and the molecular mechanism of the inhibition effect may be down-regulation of cyclin D1 expression.
关键词
肺癌
细胞增殖
基因表达
癌细胞转移
NF-kappa B
lung neoplasms
cell proliferation
transfection
gene expression
