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H6N1亚型禽流感病毒A/Mallard/Heilongjiang/301/2007非结构蛋白基因全序列扩增及分析 被引量：1

Amplification and Analysis of the Nucleotide Sequence of the non-structural Protein Gene of Subtype H6N1 Avian Influenza Virus A/Mallard/Heilongjiang/301/2007
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摘要 2007年秋我们从迁徙水禽绿头鸭(Anas platyrhynchos)中分离出一株H6N1亚型低致病性禽流感病毒A/Mallard/ Heilongjiang/301/2007。通过设计特异性引物对其非结构蛋白基因(NS)进行了全序列的扩增,并在GenBank中的序列进行比较,发现A/Mallard/Heilongjiang/301/2007属于欧亚系禽流感病毒,此株病毒NS基因与duck/Hokkaido/Vac-1/ 2004 H5N1株NS基因同源率高达98.5%,可能与北海道地区禽流感病毒具有共同起源。另外此株病毒NS基因在263～277位并没有发生15碱基的缺失,所以应属于等位基因A,亚系Ⅰ型。 A low pathogenic H6N1 avian influenza virus strain A/Mallard/Heilongjiang/301/2007 was isolated from a migratory mallard （Anas platyhynchos ） in Autumn, 2007 .The non - structural protein （NS） gene was amplified With a pair of primers and its DNS sequence was determined. Sequence comparison in GeneBank indicated that this virus A/Mallard/Heilongjiang/301/2007 belonged to EuroAsian sublineage., homology of NS gene of this virus With NS gene of duck/Hokkaido/Vac - 1/2004 H5N1 was as high as98.5%, so they have a possible common origin. No deletion of a 15 nucleotide fragment at 263 - 277 site of NS gene was observed, indicating that it belonged to Allele A sublineage Ⅰ .
作者 李晓冰 华育平 张进
机构地区 东北林业大学野生动物资源学院
出处 《野生动物》 2008年第3期135-137,共3页
基金 野生鸟类疫源疫病监测国家林业局科技项目
关键词 野禽源 禽流感 非结构蛋白 Avian influenza H6N1 Mallard Non- structural protein gene DNA sequence
分类号 Q953 [生物学—动物学]
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参考文献8

  • 1Qiu Y,Krug R M.The influenza virus NS1 protein is a poly[A]-binding protein that inhibits nuclear export of mRNAs containing poly[A].Journal of Virology,1994,68:2425-2432.
  • 2Enami M,Enami K.Characterization of influenza virus NS1 protein by using a novel heper-virus-free reverse genetic system.Journal of Virology,2000,74:5556-5561.
  • 3Ludwig S,Wang X,Ehrhardt L,et al.The influenza A virus NS1 protein inhibits activation of jun N-Terminal kinase and AP-1 transcription factors.Journal of Virology,2002,76(21):11166-11171.
  • 4Jiso Yasuda,Susumu Nakada,Atsushi kato,et al.Molecular assembly of influenza virus:association of the NS2 protein with virion matrix.Virology,1993,196(1):249-255.
  • 5Cheung C L,Vijaykrishna D,Smith G J,et al.Establishment of Influenza A Virus(H6N1)in Minor Poultry Species in Southern China.Journal of Virology,2007110):10402-10412.
  • 6龙进学,薛峰,彭宜,顾敏,刘秀梵.H5N1亚型禽流感病毒NS第263~277位核苷酸缺失提高病毒对鸡的致病力[J].微生物学报,2006,46(2):301-305. 被引量：11
  • 7Jiao Peirong,Tian Guobin,Li Yanbing,et al.A Single-Amino-Acid Substitution in the NS1 Protein Changes the Pathogenicity of H5N1 Avian Influenza Viruses in Mice.Journal of Virology,2008,(2):1146-1154.
  • 8Webster R G,Bean W J,Gorman O T,et al.Evolution and ecology of influenza A viruses.Microbiol.Rev,1992,56:152-179.

二级参考文献5

共引文献10

同被引文献13

  • 1Zell R, Krumbholz A, Eitner A, et al. Prevalence of PB1-F2 of influenza A viruses[J]. J Gen Virol, 2007, 88:536-546.
  • 2Zamarin D, Ortigoza M B, Palese P. Influenza A virus PB1-F2 protein contributes to viral pathogenesis in mice[J]. J Virol, 2006,80 (16) : 7976-7983.
  • 3McAuley J L, Hornung F,Boyd K L,et al. Expression of the 1918 influenza A virus PB1-F2 enhances the pathogenesis of viral and secondary bacterial pneumonia[J].Cell Host Microbe,2007(2) :240-249.
  • 4Mazur I, Anhlan D, David M, et al. The proapoptotic influenza A virus protein PB1-F2 regulates viral poly- merase activity by interaction with the PB1 protein [J].Cell Microbiol,2008,10(5) :1140-1152.
  • 5Chen G W, Yang C C, Tsao K C, et al. Influenza A virus PB1-F2 gene in recent Taiwan isolates[J]. Emerg Infect Dis, 2004,10 (4) : 630-636.
  • 6Olsen B, Munster V J, Wallensten A, et al. Global pat- terns of influenza A virus in wild birds[J]. Science, 2006,312:384-388.
  • 7Webster R G,Bean W J,Gorman O T,et al. Evolution and ecology of influenza A viruses[J]. Microbiol Rev, 1992,56(1) :152-179.
  • 8Pancuchdrovd H,Russ G. PB1-F2 gene in influenza a viruses of different hemagglutinin subtype [J]. Acta Virol,2006,50(4) :269-272.
  • 9Yamada H, Chounan R, Higashi Y, et al. Mitochondrial targeting sequence of the influenza A virus PB1-F2 protein and its function in mitochondria[J]. FEBS Lett, 2004,578 : 331-336.
  • 10Bruns K, Studtrucker N, Sharma A, et al. Structural characterization and oligomerization of PB1-F2, a proapoptotic influenza A virus protein[J]. J Biol Chem, 2007,282( 1 ) : 353-363.

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野生动物
2008年 第3期

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