亚慢性砷染毒对大鼠肾小球细胞半胱氨酸蛋白酶-3表达的影响

Expression of Caspase-3 in Renal Glomerulus Cells in Rats with Sub-chronic Arsenic Poisoning

目的探讨半胱氨酸蛋白酶-3(caspase-3)对砷中毒肾小球细胞凋亡的影响。方法将清洁级SD大鼠随机分为高、低剂量组和对照组(自来水),每组10只,雌雄各半,将As2O3溶于饮水中进行染毒。高、低剂量组砷染毒剂量分别为10、0.4mg/(kg·d),对照组可自由饮水,连续染毒4个月,每周称体重。染毒结束后,测定尿砷、血砷的含量,免疫组化SABC方法检测肾小球细胞caspase-3的表达,并进行图像分析、计数。结果与对照组比较,高、低剂量组尿砷、血砷含量均较高,肾小球细胞caspase-3阳性细胞数均较多,灰度值均较低;与低剂量组比较,高剂量组尿砷、血砷含量均较高,肾小球细胞caspase-3阳性细胞数较多,灰度值较低,差异均有统计学意义(P<0.01)。结论慢性砷中毒所致肾小球细胞凋亡可能与肾小球细胞caspase-3的表达明显增加有关。

Objective To investigate the influence of caspase-3 on the apoptosis of glomerulus cells in chronic arsenic poisoning. Methods Sprague-Dawley（SD） rats in cleanliness grade were randomly divided into high-dose group, low-dose group and control group, 10 in each （5 males and 5 females）. The rats in high and low groups were treated with As203 through drinking water, 10 and 0.4 mg/（kg, d） respectively, for four months. The content of arsenic in the blood and urine was determined. The expression of caspase-3 in the glomerulus cells was detected by SABC immunohistochemistry and analyzed through image patterns. Results Compared with the control group, a higher arsenic content in the blood and urine, more positive cells of caspase-3 and lower OD value the glomerulus cells were found in both of high-dose and low-dose groups. Compared with low-dose group, a higher arsenic content in the blood and urine, more positive cells of caspase-3 and the lower value of grey degree in the renal glomerulus cells were all found in high-dose group. Conclusion The obvious increase of caspase-3 in the glomerulus cells may play a role in the apoptosis of the glomerulus cells induced by chronic arsenic poisoning.