300L气升式反应器高密度培养生产PHB实验

FED-BATCH CULTRUE OF Erwinia carotovora IN A 300L AIR-LIFT BIOREACTOR

在３００Ｌ气升式反应器中，用欧文氏工程菌Ｅｃｃ１３Ｂ－１，以工业蔗糖为碳源，采用流加补料方式，进行高密度培养生产ＰＨＢ实验培养时间２５ｈ，最大干细胞浓度达４３９５ｇ／Ｌ。

Experiment was successfully carried out to produce poly β hydroxybutyric acid(PHB)by fed-batch culture of recombinant Erwinia carotovora Fermentation was performed for 25 h in a 300 L air-lift bioreactor using industrial sucrose as carbon source The maximum dry cell weight was 43 95 g/L,PHB content was 50 02% The air-lift bioreactor showed the suitability for large scale high cell density fermentation in producing PHB