HPLC-MS法测定不同产地冻干鲜鱼腥草中黄酮类成分的含量

Quantitative analysis of flavonoids in freeze-dried Houttuynia cordata from different habitats by HPLC-MS

目的考察不同产地冻干鲜鱼腥草黄酮类活性成分的含量。方法采用HPLC-MS法，Alhima C18柱；流动相：体积分数为0．2％的冰醋酸溶液为流动相A，乙腈为流动相B，梯度洗脱：0—20min，5％-20％B；20—40min，20％-20．5％B；40—50min，20．5％-35％B；流速：0．6mL／min；柱后分流：0．4mL／min进质谱仪；UV和MS同时检测。结果香港产的鱼腥草5个黄酮类成分含量都低于四川峨嵋和广东产地，四川峨嵋产地的芸香苷、金丝桃苷、槲皮苷含量均高于广东产的，而广东产地的槲皮素-3-O-β-D-半乳糖-7-O-β-D-葡萄糖苷和槲皮素-3-O－α－L-鼠李糖-7-O-β-D－葡萄糖苷含量高于四川峨嵋产地。结论不同产地冻干鲜鱼腥草的黄酮类活性成分含量不同。

Objective To compare the contents of flavonoids in freeze-dried Houttuynia cordata from different habitats. Methods Samples were analyzed by HPLC-MS using an Alhima C18 column. The mobile phase consisted of 0. 2% acetic acid in water （A） and acetonitrile （B） using a gradient elution program of 20% （B） in 0 -20 min, 20% -20.5% （B） in 20 -40 min, 20.5% -35% （B） in 40 -50 min. The flow rate was 0. 6 mL/min. Results The contents of five flavonoids in HongKong were higher than those in Sichuan and Guangdong Province. The contents of rutin, hyperoside and quercitroside in Sichuan Province were higher than those in Guangdong Province, while the contents of quercetin 3-O-β-D-galactopyranosyl-7-O-β-D-glucopyranoside and quercetin 3-O-L-rhamnopyranosyl-7- O-β-D-glucopyranoside in Guangdong Province were higher than those in Sichuan Province. Conclusion There are great differences in the contents of flavonoids from different habitats.