死精子症精子相关蛋白的蛋白质组学研究

Proteomic Identification of Necrozoospermia-related Proteins

目的:运用双向电泳(2-DE)和质谱技术比较1例死精子症患者和正常人精子的蛋白质组成差异。方法:用2-DE分离了10例正常生育者30份精子标本和1例死精子症3份精子标本的蛋白质,分别获得其参考图谱并进行比较,用质谱技术鉴定其中部分差异蛋白点。结果:在正常精子图谱和死精子症患者精子图谱分别分离出(905±57)和(792±28)个蛋白质点,死精子症患者精子图谱上有178个点未能匹配。对其中6个在患者图谱中缺失的蛋白质点进行了鉴定。结论:死精子症患者图谱上缺失的6个蛋白,可能与死精子症的形成有关。

Objective： To compare the proteomic differences between normal and necrozoospermic human spermatozoa. Methods： We isolated proteins from 30 ejaculates of 10 healthy donors and 3 specimens of a necrozoospermia patient by two-dimensional gel electrophoresis （2-DE）, analyzed their 2-DE maps and identified some differentially expressed proteins by mass spectrometry. Results ： A total of （905 ± 57 ） and （ 792 ± 28 ） proteins were isolated from the spermatozoal maps of the normal and necrozoospermic men, respectively, and 178 found to be differentially expressed in that of the necrozoospermia patient. The 6 missing protein spots in the necrozoospermic map were identified as sperm protein （ human, accession No. 060904）, zinc finger protein 174 （ AW-1 ）, F-actin capping protein alpha-3 subunit, testis-specific inhibitor of apoptosis, death domain receptor 3 soluble form （fragment） and peptide similar to the activator of CREM in the testis. Conclusion ： Six missing protein spots were identified in the necrozoospermic spermatozoal map which may be associated with the development of necrozoospormia.