抗人轮状病毒牛血丙球蛋白的研制

Preparation of γ-globulin oral liquid against human rotovirus from fattening cattle blood

目的通过牛源抗人轮状病毒免疫球蛋白的研究,并经胃肠道给药观察其对人工感染仔猪腹泻的治疗效果。方法以人轮状病毒(HRV"Wa"株)为抗原免疫接种健康育肥黄牛,制备成牛血丙球蛋白口服液。结果丙球蛋白含量达52mg/ml,纯度达80%,中和抗体效达1∶16384,初步研究表明对人工感染HRV"Wa"毒株发生腹泻的仔猪治疗有效率达92.3%。结论牛源抗人轮状病毒免疫球蛋白可能成为新型治疗轮状病毒引起腹泻的有效新药。

Objective The treatment of dian＇hea was observed through anti -human rotavirus bovine immunoglobulin studies and the administrating gastrointestinal tract of infected pigs. Methods The γ- globulin oral liquid was prepared from blood of healthy fattened oxen which had been vaecined by antigen of human rotovirus （ HRV - Wa）. Results lml of the γ- globulin oral liquid contained 52mg 7 - globulin and had 80% of purity and its neutral antibody efficiency reached to 1：16384. The initial studies show that the oral liquid was used to treat piglets suffering from diarrhea easued by HRV - Wa and had a cure rate of 92.3%. Conclusion Bovine immunoglobulin anti - human rotavirus infection has the possibility to be a new type of medicine which is efficient to treat human rotavims infection.