摘要
目的:比较耐力游泳训练和停训对大鼠白色脂肪组织PPARγ蛋白表达的影响,探讨停训对脂肪细胞增殖与分化的调控作用。方法:SD纯系雄性大鼠随机分为12周对照组(C12)、12周训练组(E12)和8周训练后停训4周组(DE)组,E12组和DE组大鼠进行无负重游泳训练。流式细胞仪技术检测大鼠脂肪组织中PPARγ及脂肪细胞增殖分化情况。结果:DE组大鼠内脏脂肪垫重量显著低于C12组,高于E12组;E12组大鼠的PPARγ蛋白表达量和脂肪细胞增殖比例均较相应C12组有显著增加;DE组的PPARγ蛋白表达量及脂肪细胞增殖分化与C12组及E12组无显著性差异。结论:系统游泳训练可促进生长发育期大鼠白色脂肪组织PPARγ蛋白表达量的增加,从而对脂肪细胞增殖分化的增加产生一定影响;大鼠游泳训练后的停训可增加其白色脂肪组织PPARγ蛋白表达量和脂肪细胞增殖分化比例,有内脏脂肪积累增多而表现出肥胖的倾向。
This paper compares the effect of endurance swimming exercise and detraining on the expression PPAR gamma and discuss whether can affect the adipose differentiation. Male SD rats were randomly divided into control (C12) and exercise-trained (El2) and detraining (DE) group. Rats in the El2 group were swimming for 12 weeks. The DE group is combined exercise-trained for 8 weeks and detraining for 4 weeks. The level of PPAR gamma protein and the percent of adipose cell multiplication were detected by flow cytometric analysis. The result shows that the weight of adipose tissues in DE group is lower than C12, while higher than El2 group; the level of PPAR gamma protein and the percent of adipose cell multiplication in E12 group has disparity than C12,which in DE group has no disparity than C12 and E12. It concludes that detraining can promote the fat accumulation, and this effect may be induced by the change of PPAR gamma expression.
出处
《体育科学》
CSSCI
北大核心
2008年第4期48-51,64,共5页
China Sport Science
基金
河北省自然科学基金项目(C2005000156)
关键词
耐力游泳训练
脂肪细胞分化
PPARΓ
鼠
动物实验
endurance swimming training
adipose differentiation
PPARy
rat
animal experiment