模拟4 000m高住低练对大鼠肝细胞凋亡与增殖的影响

Effects of Simulated 4000m Hi-Lo Training on Liver Apoptosis and Proliferation of Rats

目的:揭示"高住低练"中肝细胞凋亡和增殖的情况及之间的关系。方法:60只健康成年雄性SD大鼠随机分为正常对照组(C)、8h低氧暴露组(8HE)、12h低氧暴露组(12HE)、常氧运动组(T)、8h高住低练组(8Hilo)和12h高住低练组(12Hilo)。采用TUNEL.法检测细胞凋亡和免疫组织化学法观察PCNA蛋白表达。结果:两个高住低练组大鼠的凋亡发生率及凋亡指数显著高于低氧暴露组和常氧运动组大鼠(P<0.01);12h低氧暴露组大鼠细胞增殖指数显著低于8h低氧暴露组且均低于正常对照组(P<0.01),与正常对照组相比,训练各实验组均有所增加(P<0.05),高住低练组比低氧暴露组明显增加(P<0.01);单纯低氧暴露时,凋亡与增殖之间呈负相关,而训练介入后,高住低练过程中凋亡与增殖之间呈现正相关。结论:高住低练对肝细胞凋亡的影响比单纯低氧暴露和常氧运动中大;高住低练过程中肝细胞凋亡与增殖呈正相关,一定程度下,运动有利于肝组织细胞的恢复。

This research tries to study the connection between liver apoptosis and proliferation. 60 male SD rats were randomly divided into Group C, 8hHE, 12hHE, T, 8Hilo and 12Hilo. Apoptosis was detected in paraffin sections by the TUNEL. Expression of PCNA protein was examined by immunohistochemistry. The result shows that the indexes of apoptosis occurred in groups Hilo more than groups HE and T （P〈0. 01）. PCNA protein in group 12hHE expressed obviously lower than group 8hHE （P〈0. 01）. As compared with group C, PCNA protein expressed slightly increases in spite of groups HE, group T and groups Hilo. And as compared with groups HE, it showed that there was prominent significance in groups Hilo （P〈0.01）. It concludes that liver apoptosis occurred in group 12hHE and 12Hilo more than in group 8hHE and 8Hilo, and demonstrates correlativity between liver apoptosis and proliferation.