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酶抗体应用实现热启动PCR 被引量：3

Using Antibodies Against Taq-FS Polymerase for Hot-start PCR
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摘要 通过制备酶抗体,形成抗原抗体复合物,使得酶在较高的温度95℃,1min,得以释放发挥聚合活性,实现热启动PCR.建立的热启动PCR体系灵敏度高,可以检测到1个拷贝的DNA分子,较普通PCR体系提高了3个数量级,并且具有较好的线性关系.这种通过形成抗原抗体复合物达到热启动PCR目的的方法的建立,具有着重要的意义,是提高PCR灵敏度和特异性的重要方法之一. Hot-start PCR is advantageous for its higher speciality, sencitivity and linear amplification. We have used an antibodies against Taq-FS polymerase for the setup hot-start PCR reactions. The antibody used could block the activity of Taq-FS polymerase at low tempreture （or below）. When heated at high temperature 95℃, 1 min, the antibody was denatured and the activity of Taq-FS polymerase was released. Our hot-start PCR system was capable of detecting as low as one copy of template DNA.
作者 林晴 许木于 李庆阁
机构地区 福建中医学院 厦门大学生命科学学院
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2008年第5期475-480,共6页 Chinese Journal of Biochemistry and Molecular Biology
关键词 热启动 抗体 PCR hot-start antibody PCR
分类号 Q503 [生物学—生物化学]
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参考文献21

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共引文献1

同被引文献25

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引证文献3

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中国生物化学与分子生物学报
2008年 第5期

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