中国皮划艇耐力运动员线粒体高变区Ⅱ序列多态性与最大有氧能力的关联分析

The Association Analysis between Maximal Aerobic Capability and Sequence Polymorphisms of Mitochondrial DNA Hypervariable RegionⅡ in Chinese Endurance Canoeing Athletes

目的:探讨线粒体基因高变区Ⅱ(mtDNA HVR-Ⅱ)与有氧运动能力的关联性。方法:国家皮划艇集训队汉族耐力运动员94人为耐力组;随机选取年龄、地域相当的汉族大学生92人为普通对照组。对所有受试者采用PCR扩增、测序确定mtDNA HVR-Ⅱ的序列多态性,采用跑台与气体代谢法测定最大摄氧量。结果:我国汉人和耐力运动员mtDNA HVR-Ⅱ的np150、np191-192、np194、np195和np317位点多态性在最大摄氧量上发现显著性差异。对照组女性np150位点C基因型VO2max/kg显著高于T基因型(P<0.05);耐力组男性np191-192位点"插入A"基因型的VO2max/kg显著高于"无插入"基因型(P<0.05);耐力组男性np194位点T基因型VO2max/kg显著高于C基因型(P<0.05);耐力组男性np195位点C基因型VO2max显著高于T基因型(P<0.05);对照组男性np317位点C基因型VO2max/kg高于G/C基因型(P<0.05)。结果提示:mtDNAHVR-Ⅱ的np150、np191-192、np194、np195和np317多态性与最大摄氧量可能关联,有可能成为人类运动能力的遗传标记。

Objective To investigate the association between mitochondrial DNA hypervariable Region Ⅱ （mtDNA HVR-Ⅱ ）polymorphisms and aerobic capability. Methods All subjects were Han nationality,including 94 endurance canoeing athletes and 92 healthy controls. Sequence polymorphisms of mtDNA HVR-Ⅱ was determined by polymerase chain reaction（PCR）and direct sequencing. Maximal aerobic capability was measured breath by breath in treadmill excecise. Results The subjects with ＂C＂ gene type in female controls had significantly higher VO2max/kg at np150（P〈0.05） ;the subjects with ＂insert A＂ gene type in male endurance group had significantly higher VO2max/kg at np191-192（P〈0.05）;the subjects with ＂T＂ gene type in male endurance athletes had significantly higher VO2max/kg at np194（P〈0.05） ;the subjects with ＂C＂ gene type in male endurance athletes had significantly higher VO2max at np195 （P〈0. 05） ;the subjects with ＂C＂ gene type in male controls had significantly higher VO2max/kg at np317（P〈0.05）. The results suggested that np150, np191-192, np194, np195 and np317 in mtDNA HVR Ⅱ might be related to VO2max,VO2max/kg,which probably used as gene markers for human endurance performance.