水通道蛋白1在肺腺癌组织中的表达和分布

Expression and distribution of aquaporin 1 in the adenocarcinoma of lung

目的:观察水通道蛋白1(AQP1)在肺腺癌组织中的表达并分析其临床意义。方法:取肺腺癌组织30例及正常肺组织30例,应用免疫组织化学,Western blot技术检测AQP1蛋白在肺腺癌和正常对照组织中的表达及分布,并收集临床资料,分析其与临床病理资料关系。结果:AQP1散在表达于正常肺组织的毛细血管内皮细胞,在肺腺癌组织中AQP1大量表达于肿瘤的血管内皮细胞以及肿瘤细胞膜、细胞浆;肺腺癌中AQP1蛋白表达水平较正常组织明显增多,二者之间差异有统计学意义;AQP1在肺腺癌组织中表达与肿瘤病理分级、淋巴结转移相关(P<0.05),而与肺腺癌的TNM分期无明显相关性(P>0.05)。结论:AQP1在肺腺癌组织中表达增高,在正常组织中较低。提示AQP1可能与肺腺癌的发生和发展密切相关,具体机制有待深入研究。

Objective：To detect the expression and distribution of aquaporinl（AQP1） in adenocarcinoma of the lung and to analyze its clinical significance. Methods：The lung tissue of 30 cases of pulmonary adenocarcinoma and 30 normal cases were sampled immediately after surgery to study the expression and distribution of aquaporinl in adenocarcinoma of the lung by immunohistochemical and Western blot analysis. Results：The sporadic expression of AQP1 was found in the vascular endothelial cells of normal lung tissues. The majority of AQP1 expression could be detected in the vascular endothelial cells,tumor cells in adenocarcinoma of the lung;the expression of AQP1 protein in tumor tissues was remarkably higher than that in normal tissues beside tumors;the expression of AQP1 protein in tumor tissues was correlated with tumor grading and lymph node metastasis. Conclusion：The expression of AQP1 was increased in adenocarcinoma of the lung over normal lung tissues. It suggested that AQP1 might play an important role in the formation and development of adenocarcinoma of lung and the mechanism need to be further studied.