LRP16基因在肺癌中的表达及其临床意义

Clinical significance and prognostic value of the expression of LRP16 gene in lung cancer

目的研究肺癌组织中LRP16基因的蛋白表达水平及其临床病理意义。方法收集54例原发型肺癌患者手术切除的新鲜组织标本和部分临床病理资料,用Westernblot方法检测肿瘤组织及癌旁正常肺组织中LRP16蛋白的表达水平,并分析其与临床病理资料间的联系。以肿瘤组织LRP16蛋白的表达较癌旁正常肺组织高2倍为LRP16过表达的标准。结果54例肺癌标本中有15例LRP16过表达,过表达率约27.8%(15/54)。23例肺腺癌中有11例(47.8%)LRP16过表达,27例肺鳞癌中有4例(14.8%)LRP16过表达,二者比较差异显著(P<0.05)。在2例大细胞癌和2例小细胞癌中,LRP16均呈极低表达或未表达。肿瘤直径>3cm的10例仅2例(20.0%)LRP16过表达,直径=3cm的44例中有13例(29.5%)LRP16过表达,二者比较无显著性差异(P>0.05)。有肺门或(及)纵隔淋巴结转移的20例中有10例(50.0%)LRP16过表达,而无转移的34例中仅5例(14.7%)LRP16过表达,二者比较差异显著(P<0.05)。结论LRP16基因在肺腺癌组与鳞癌组、有淋巴结转移组与无淋巴结转移组表达均有较大差异,提示其不仅是一个肺癌相关基因,而且可能与肺癌的分子分期相关。

Objective To investigate the expression of LRP16 gene in lung cancer, and explore its clinicopathological significance. Methods Fresh resected tissues from 54 patients with primary lung cancer were collected and the clinicopathological data were gathered. The expression of LRP16 protein in cancer tissues and the matched normal tissues were determined by Western blotting, and the relationship between LRP16 expression and clinicopathological data was analyzed. It was defined as overexpression when the LRP16 expression of cancer tissues was twice or more higher than that of matched normal tissues. Results The LRP16 was overexpressed in 15 out of 54 patients with lung cancer （27.8%）. Among the 23 patients with adencarcinoma, the overexpression of LRP16 was found in 11 cases （47.8%）, while in the patients with squamous carcinoma, the overexpression of LRP16 was only found in 4 out of 27 cases （14.8%）, and there was a significant difference between the two groups （Pearson test, P=0.0258）. Besides, very low expression or non-expression of LRP16 was found in 2 large cell lung cancer and 2 small cell lung cancer. The overexpression rate of LRP16 was 20.0% （2/10） in tumor with diameter less than 3cm and 29.5% （13/44） in tumor with diameter ≥3cm, and there was no significant difference between the two groups （Pearson test, P=0.7224）. Conclusions There were significant differences of LRP16 overexpression in cases of adencarcinoma or squamous carcinoma with or without lymphatic metastasis. It is suggested that LRP16 is a tumor-related gene of lung cancer, and may play an important role in molecular staging of lung cancer.