摘要
An insect excitatory toxin gene from Buthus martensii Karsch(BmKIT)was cloned into the expres-sion vector,pET-28a.BmKIT was expressed as inclusion bodies in Escherichia coli BL21(DE3)hostcells.The authenticity of in vitro expressed protein was confirmed by Western blot.The inclusion bodyprotein band in SDS-PAGE was excised and the protein,BmKIT,was extracted.Polyclonal antibodies tothe purified protein were raised in rabbits.The antibody reacted specifically with the expressed BmKITand was used to quantify its presence in transgenic cotton.
An insect excitatory toxin gene from Buthus martensii Karseh （BmKIT） was cloned into the expression vector, pET-28a. BmKIT was expressed as inclusion bodies in Eseherichia coli BL21 （DE3） host cells. The authenticity of in vitro expressed protein was confirmed by Western blot. The inclusion body protein band in SDS-PAGE was excised and the protein, BmKIT, was extracted. Polyelonal antibodies to the purified protein were raised in rabbits. The antibody reacted specifically with the expressed BmKIT and was used to quantify its presence in transgenic cotton.
基金
the National Natural Science Foundation of China(No30670282 and 30470239)
Natural Science Foundation of Shanxi Province(20051065 and 20041033)
Scholar Foundation of Shanxi Province
关键词
钳蝎
神经毒素
原核
抗体
Buthus martensii, neurotoxin, prokaryotic expression, antibody preparation, transgenic cotton
