氧化损伤星形细胞与骨髓基质细胞混合培养的形态学研究

The morphology research on cocultivation of H2O2-injuryed astrocytes and bone marrow stromal cells

目的观察氧化损伤星形细胞与骨髓基质细胞（MSCs）混合培养时星形细胞恢复及存活的形态学变化，以进一步探讨MSCs修复脑损伤的机制。方法将氧化损伤后的第2代星形细胞与第3代MSCs混合培养作为实验组，分别取上述两种细胞在相同条件下单独培养作为对照组。光镜及电镜观察损伤星形细胞的恢复及存活情况。结果实验组损伤星形细胞的恢复及两种细胞的存活情况明显优于对照组。结论将损伤星形细胞与MSCs混合培养可以促进星形细胞恢复，并提高两种细胞的存活能力。MSCs修复脑损伤机制可能与其促进损伤星形细胞恢复、减少星形细胞死亡有关。

Objective To observe the self-repair and survive ability of H2O2-injuryed astrocytes when cocuhuring with bone marrow stromal cells（MSCs） and to further analyze the role of MSCs in central nervous system injury repairing. Method Experimental group： H2O2-injuryed passage 2 astrocytes were cocultured with passage 3 MSCs. Control groups： these two kinds of cells were cultured respectively under the same condition. After 3 days and 7 days cultivation, observe the morphology changes under light microscope and electron microscope. Results After 3 days and 7 days cultivation, the astrocytes and MSCs in experimental group grew well compared with control groups. Conclusions Coculturing of astrocytes with MSCs can promote the self-repairing ability of astrocytes and increase astrocytes survival post H2O2-induced injury. The therapeutic benefit MSCs provided to injuryed brain may be in part due to the MSCs conferred protection of astrocytes.