复制型HBV转基因小鼠遗传稳定性研究

The Genetic Stability of Replicating HBV Transgenic Mice

目的：提高复制型HBV转基因小鼠的遗传稳定性。方法：应用回交传代及双杂交育种法，经荧光定量PCR、ELISA和化学发光法研究HBV基因在小鼠体内的复制与表达。结果：HBV转基因小鼠已稳定传至第23代，血清HBsAg达4122．31±2044．74IU／ml，93．93％的转基因小鼠血清HBVDNA达10^4-10^4copies／ml，表达复制水平较早期有显著提高并稳定传代；雌雄小鼠之间表达水平无显著性差异。结论：该转基因小鼠经过培育传代，已成为一个高表达且遗传稳定的复制型HBV小鼠模型。

Objective：To improve the genetic stability of HBV gene in transgenic mice. Methods： HBV transgenic mice were bred by backcross and double cross. The HBV gene expression and replication were studied with real-time PCR,ELISA and chemiluminescence. Results：The HBV transgenic mice have stably bred to 23^rd generation. The serum HBsAg level is 4122.31 ±2044.74IU/ml; The rate of HBV transgenic mice whose serum HBV DNA reach 10^4 - 10^6copies/ml was 93. 93%. The HBV replication and expression were improved markedly. There is no difference between male and female mice about serum HBsAg level. Conclusion：After breeding the HBV gene was expressed stably with high-level in transgenic mice.