微生物脂肪酶的重组表达

Research Advance in Recombinant Expression of Microbial Lipases

微生物脂肪酶在传统和新型工业催化领域中的应用越来越广泛与深入。作为脂肪酶规模化制备主要途径的高效重组表达,为脂肪酶催化剂的最终形成及工业催化奠定了坚实的技术基础。概述并讨论了微生物脂肪酶重组表达的最新策略和发展趋势,阐述密码子优化、融合共表达、杂合启动子、同源高效表达、细胞表面展示和表达文库的高通量筛选等技术特点及其表达应用现状,指出细胞表面展示表达和表达文库高通量筛选体系为脂肪酶重组表达注入强劲活力;在此基础上对几种代表性微生物脂肪酶的重组表达进展作一综述,为微生物脂肪酶的重组表达研究及工业生产提供借鉴。

Microbial lipases have been widely used in traditional industries, as well as in emerging biocatalysed areas owing to their ability to catalyze a variety of reactions in aqueous and non-aqueous media. Therefore, it is very important to enhance amount of lipase production by recombinant overexpression for meeting market demand. A critical review of main and novel strategies which have been employed for recombinant expression of microbial lipases are presented, including codon optimization, fusion and co-expression, dual expression system based on hybrid promoters, homologous overexpression, cell surface displaying and high- throughput screening based on gene library of expression. These new technologies are gradually coming to the forefront in the recombinant expression of lipase, especially for cell surface displaying and high-throughput screening based on gene library of expression. Meanwhile, several recombinant expressions for representative microbial lipases were also introduced and discussed, which are available for consultation when attempting to overexpress any lipases by scientists and industrialists.