哮喘患儿激素治疗后TH1/TH2的变化

The effect of glucocorticoids on TH1/TH2 in asthma

目的观察哮喘患儿血清中白细胞介素IL12、IL13和IgE水平的变化及糖皮质激素对其的影响。方法采用ELISA法分别检测哮喘患儿急性期及口服强的松5～7天后、健康对照组血清中IL12、IL13和IgE的水平。结果哮喘组患儿IL12水平急性发作期较治疗后和健康对照组低,差异均有显著性(P<0.01)。IL13水平急性发作期较治疗后和健康对照组高,差异均有显著性(P<0.01),IgE水平急性发作期较治疗后和健康对照组高,差异均有显著性(P<0.01)。直线相关分析表明,血清中IL12与IL13负相关,与IgE呈负相关,IL13与IgE呈正相关。结论哮喘患者血清中IL13、IgE水平升高,IL12水平下降,3者的变化相关,糖皮质激素可使IL13水平下降,IL12水平升高。

Objective：To observe the changes of serum interleukin（IL）12,IL 13 and IgE in infant with asthma and to evaluate the influence of glucocorticoid on them.Methods：The serum samples were obtained from（1） Patients with asthma before and after five-seven days course of oral prednisone;（2） Healthy volunteers serum IL 12,IL13 and IgE were determined with sandwich ELISA in all patients.Results：（1）Serum level of IL12 in asthma group was significantly lower than that after treatment and normal control group（P〈0.01）;（2）in asthma group IL 13 level was significantly higher than that after treatment and normal control group（P〈0.01）;（3）Serum level of IgE in asthma group was significantly higher than that after treatment and normal control group（P〈0.01）;（4）Correlation analysis showed that the serum level of IL12 was negatively correlated to serum level of IL 13 and IgE;the serum level of IL13 was positively correlated to IgE.Conclusion：The secretion of IL12 and IL 13 was impaired in asthma with a significant increase in serum level of IL 13 and decrease in serum level of IL12.Glucocorticoid could down regulate the serum level of IL13 and up regulate the serum level of IL12,redress the imbalance of IL 12/IL 13.