限制进食刺激对肠上皮淋巴细胞数量和杀伤活性的影响

EFFECTS OF FOOD RESTRICTION STRESS ON THE NUMBERS AND CYTOTOXICITY OF IEL IN MICE

通过对限制进食刺激影响消化道肠上皮淋巴细胞及细胞亚群数量和杀伤活性的研究，发现限制小鼠进食２ｄ后，ＩＥＬ总数明显下降，并随限制进食时间的延长其总数下降更为明显。流式细胞分析仪检测结果显示，在给予刺激２ｄ，αβ－ＩＥＬ百分比明显下降，而γδ－ＩＥＬ百分比却有所上升；限制进食５ｄ后，二种细胞亚群百分比均较对照组降低。ＩＥＬ杀伤活性在限制进食２ｄ时略有升高，以后下降，限制进食５ｄ实验组ＩＥＬ杀伤活性明显低于对照组。

The effects of food restriction stress on numbers and cytotoxicity of intestinal intraepithelial lymphocytes(IEL)were investigated.Results showed that the total number of IEL reduced in 2 days after food restriction stress and became even less with prolongation of food restriction stress.Analysis by flow cytometry demonstrated that the percentage of αβ IEL decreased and that of γδ IEL increased on the second day of food restriction stress.But the percentages of both αβ IEL and γδ IEL were decreased compared with control group on the fifth day of the stress.The cytotoxicity of IEL increased on the second day of food restriction stress,then decreased,and were significantly lower than that of control group on the fifth day of food restriction stress.